Assessment of perfluoroalkyl substances in placenta by coupling salt assisted liquid-liquid extraction with dispersive liquid-liquid microextraction prior to liquid chromatography-tandem mass spectrometry

The widespread use of perfluoroalkyl substances (PFAS) is resulting in a broad human exposure to these endocrine disrupting chemicals (EDCs), prompting biomonitoring research evaluate its magnitude and impact, especially during critical windows such as fetal perinatal periods. This study was focused on developing method determine 10 PFAS placental tissue by combining salt-assisted liquid-liquid extraction with dispersive microextraction using liquid chromatography-tandem mass spectrometry. Chemometric strategies were applied optimize the experimental parameters. limit quantification 0.02 ng g?1 for all analytes, inter-day variability (as relative standard deviation) ranged from 7.9% 13.8%. Recoveries 88.2% 113.9%. suitableness procedure demonstrated assessing targeted compounds 20 placenta samples. highest concentrations recorded perfluorooctanoic acid perfluorooctane sulfonate, maximum 0.62 1.02 median 0.13 0.53 g?1, respectively. Median other detected values 0.08 g?1. analytical yields useful data PFAS.